Essential guide to reading biomedical papers:recognising and interpreting best practice
Other author
Langton, Philip D.
Type of publication
Books
Language
eng
Physical description
xx, 331 p. : ill. (some col.) ; 25 cm.
Publisher
Wiley-Blackwell, 2013
ISBN
9781119959977 9781119959965
Сall number
R850 .E85 2013
Location
Medical library
Bibliography
Includes index
Subject
biological science disciplines, education biomedical research
Genre
Textbooks/Оқулықтар/Учебная литература
Summary
This concise, easy-to-follow text gives an insight into core techniques and practices in biomedical research and how, when and why a technique should be used and presented in the literature. Readers are alerted to common failures and misinterpretations that may evade peer review and are equipped with the judgment necessary to be properly critical of the findings claimed by research articles. --from publisher description
Contents
Basic principles. Philosophy of science -- Ingredients of experimental design -- Statistics: a journey that needs a guide -- Cell and molecular. Organ bath pharmacology -- Small vessel myography -- Mammalian cell cultures: the example of airway eptihelial cell cultures for cystic fibrosis research -- Electron microscopy (TEM and SEM) -- Fluorescence microscopy -- Intracellular 'sharp' microelectrode recording -- Single electrode voltage-clamp (SEVC) -- Patch clamp recording -- Production of antibodies ; Immunocytochemistry and immunohistochemistry -- Immunoprecipitation (IP) -- Immunoblotting (western) -- Applications of green fluorescent protein (GFP) -- Fluorescent measurement of ion activity in cells -- Detection of exocytosis-- real time ; Viral vector transgenesis -- Polymerase chain reaction (PCR) and reverse transcription (RT)-PCR ; In situ hybridisation (ISH) -- Methods of interference (antisense, siRNAs and dominant negative mutations) -- Transcriptome analysis: microarrays -- Experimental proteomics -- In vivo/intergrative. Behavioural methodology -- Genetically modified mouse models -- Wireless recording of cardiovascular signals -- Electrical stimulation methods ; Extracellular recording ; Antidromic identification ; Antidromic identification -- Event-triggered averaging, including spike-triggered averaging -- Axonal transport tracing of CNS pathways -- Cardiovascular methods: general considerations for human studies ; Measuring cardiac output in humans ; Measuring peripheral blood flow in humans
